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IBL America ria for ang ii
Results from each participant are shown for COVID-19-negative patients with moderate AHRF, COVID-19-positive patients with moderate AHRF, and COVID-19-positive patients with severe AHRF. Bars represent the mean and SD values for plasma levels of <t>angiotensin</t> <t>II</t> <t>(ANG</t> II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), prolyloligopeptidase (POP) ( C ), angiotensin-converting enzyme (ACE) ( D ), and angiotensin-converting enzyme 2 (ACE2) ( E ). AHRF, acute hypoxic respiratory failure.
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1) Product Images from "A pilot study to assess the circulating renin-angiotensin system in COVID-19 acute respiratory failure"

Article Title: A pilot study to assess the circulating renin-angiotensin system in COVID-19 acute respiratory failure

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

doi: 10.1152/ajplung.00129.2021

Results from each participant are shown for COVID-19-negative patients with moderate AHRF, COVID-19-positive patients with moderate AHRF, and COVID-19-positive patients with severe AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), prolyloligopeptidase (POP) ( C ), angiotensin-converting enzyme (ACE) ( D ), and angiotensin-converting enzyme 2 (ACE2) ( E ). AHRF, acute hypoxic respiratory failure.
Figure Legend Snippet: Results from each participant are shown for COVID-19-negative patients with moderate AHRF, COVID-19-positive patients with moderate AHRF, and COVID-19-positive patients with severe AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), prolyloligopeptidase (POP) ( C ), angiotensin-converting enzyme (ACE) ( D ), and angiotensin-converting enzyme 2 (ACE2) ( E ). AHRF, acute hypoxic respiratory failure.

Techniques Used: Clinical Proteomics

Results from each partcipant are shown for peptide levels grouped by COVID-19-negative patients with moderate AHRF and combined moderate and severe COVID-19-positive patients with AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), ANG-(1–7):ANG II ratio ( C ), angiotensin-converting enzyme (ACE) ( D ), angiotensin-converting enzyme 2 (ACE2) ( E ), and prolyloligopeptidase (POP) ( F ). AHRF, acute hypoxic respiratory failure.
Figure Legend Snippet: Results from each partcipant are shown for peptide levels grouped by COVID-19-negative patients with moderate AHRF and combined moderate and severe COVID-19-positive patients with AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), ANG-(1–7):ANG II ratio ( C ), angiotensin-converting enzyme (ACE) ( D ), angiotensin-converting enzyme 2 (ACE2) ( E ), and prolyloligopeptidase (POP) ( F ). AHRF, acute hypoxic respiratory failure.

Techniques Used: Clinical Proteomics

Correlation between “direct” and “surrogate” ACE2 or POP activity. Results from each participant are shown and represent the direct serum ACE2 activity ( A ) and POP activity ( B ) plotted against “surrogate” enzymatic activities using the corresponding plasma ANG-(1–7):ANG II peptide ratio. Each dot represents the paired corresponding enzymatic activity and peptide ratio for an individual patient. ACE2, angiotensin-converting enzyme 2; ANG, angiotensin; POP, prolyloligopeptidase.
Figure Legend Snippet: Correlation between “direct” and “surrogate” ACE2 or POP activity. Results from each participant are shown and represent the direct serum ACE2 activity ( A ) and POP activity ( B ) plotted against “surrogate” enzymatic activities using the corresponding plasma ANG-(1–7):ANG II peptide ratio. Each dot represents the paired corresponding enzymatic activity and peptide ratio for an individual patient. ACE2, angiotensin-converting enzyme 2; ANG, angiotensin; POP, prolyloligopeptidase.

Techniques Used: Activity Assay, Clinical Proteomics



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Results from each participant are shown for COVID-19-negative patients with moderate AHRF, COVID-19-positive patients with moderate AHRF, and COVID-19-positive patients with severe AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), prolyloligopeptidase (POP) ( C ), angiotensin-converting enzyme (ACE) ( D ), and angiotensin-converting enzyme 2 (ACE2) ( E ). AHRF, acute hypoxic respiratory failure.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: A pilot study to assess the circulating renin-angiotensin system in COVID-19 acute respiratory failure

doi: 10.1152/ajplung.00129.2021

Figure Lengend Snippet: Results from each participant are shown for COVID-19-negative patients with moderate AHRF, COVID-19-positive patients with moderate AHRF, and COVID-19-positive patients with severe AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), prolyloligopeptidase (POP) ( C ), angiotensin-converting enzyme (ACE) ( D ), and angiotensin-converting enzyme 2 (ACE2) ( E ). AHRF, acute hypoxic respiratory failure.

Article Snippet: Stored plasma was thawed on ice, extracted on Sep-Pak C18 columns (Waters Corp., Milford, MA), and quantified in duplicates by radioimmunoassay (RIA) for ANG II (IBL America, Minneapolis, MN, sensitivity of 2.0 pg/mL; intra-assay and interassay coefficients of variation of 8% and 20%, respectively) and ANG-(1–7) (custom RIA, sensitivity of 2.5 pg/mL; intra-assay and interassay coefficients of variation of 12% and 22%, respectively) ( , ).

Techniques: Clinical Proteomics

Results from each partcipant are shown for peptide levels grouped by COVID-19-negative patients with moderate AHRF and combined moderate and severe COVID-19-positive patients with AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), ANG-(1–7):ANG II ratio ( C ), angiotensin-converting enzyme (ACE) ( D ), angiotensin-converting enzyme 2 (ACE2) ( E ), and prolyloligopeptidase (POP) ( F ). AHRF, acute hypoxic respiratory failure.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: A pilot study to assess the circulating renin-angiotensin system in COVID-19 acute respiratory failure

doi: 10.1152/ajplung.00129.2021

Figure Lengend Snippet: Results from each partcipant are shown for peptide levels grouped by COVID-19-negative patients with moderate AHRF and combined moderate and severe COVID-19-positive patients with AHRF. Bars represent the mean and SD values for plasma levels of angiotensin II (ANG II) ( A ), angiotensin-(1–7) [ANG-(1–7)] ( B ), ANG-(1–7):ANG II ratio ( C ), angiotensin-converting enzyme (ACE) ( D ), angiotensin-converting enzyme 2 (ACE2) ( E ), and prolyloligopeptidase (POP) ( F ). AHRF, acute hypoxic respiratory failure.

Article Snippet: Stored plasma was thawed on ice, extracted on Sep-Pak C18 columns (Waters Corp., Milford, MA), and quantified in duplicates by radioimmunoassay (RIA) for ANG II (IBL America, Minneapolis, MN, sensitivity of 2.0 pg/mL; intra-assay and interassay coefficients of variation of 8% and 20%, respectively) and ANG-(1–7) (custom RIA, sensitivity of 2.5 pg/mL; intra-assay and interassay coefficients of variation of 12% and 22%, respectively) ( , ).

Techniques: Clinical Proteomics

Correlation between “direct” and “surrogate” ACE2 or POP activity. Results from each participant are shown and represent the direct serum ACE2 activity ( A ) and POP activity ( B ) plotted against “surrogate” enzymatic activities using the corresponding plasma ANG-(1–7):ANG II peptide ratio. Each dot represents the paired corresponding enzymatic activity and peptide ratio for an individual patient. ACE2, angiotensin-converting enzyme 2; ANG, angiotensin; POP, prolyloligopeptidase.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: A pilot study to assess the circulating renin-angiotensin system in COVID-19 acute respiratory failure

doi: 10.1152/ajplung.00129.2021

Figure Lengend Snippet: Correlation between “direct” and “surrogate” ACE2 or POP activity. Results from each participant are shown and represent the direct serum ACE2 activity ( A ) and POP activity ( B ) plotted against “surrogate” enzymatic activities using the corresponding plasma ANG-(1–7):ANG II peptide ratio. Each dot represents the paired corresponding enzymatic activity and peptide ratio for an individual patient. ACE2, angiotensin-converting enzyme 2; ANG, angiotensin; POP, prolyloligopeptidase.

Article Snippet: Stored plasma was thawed on ice, extracted on Sep-Pak C18 columns (Waters Corp., Milford, MA), and quantified in duplicates by radioimmunoassay (RIA) for ANG II (IBL America, Minneapolis, MN, sensitivity of 2.0 pg/mL; intra-assay and interassay coefficients of variation of 8% and 20%, respectively) and ANG-(1–7) (custom RIA, sensitivity of 2.5 pg/mL; intra-assay and interassay coefficients of variation of 12% and 22%, respectively) ( , ).

Techniques: Activity Assay, Clinical Proteomics